Stem Cell Core

The Core isolates fibroblasts, PBMCs or other cell types from donor samples and cryopreserves these for future use.

The Core receives samples from investigators and reprograms these using non-integrating vector systems resulting in footprint free iPSCs. These samples can be any cell type and we have successfully reprogrammed cells from multiple species including humans, non-human primates and mice. Additionally, we provide a full suite of quality control options to ensure that the iPSCs produced are of the highest quality.

The Core designs and carries out gene editing of pluripotent stem cells using CRISPR/CAS9. These services include, in silico design of guide RNAs and homologous damage repair templates, validation of enzymatic cutting efficiencies, transfection of reagents into the cells, clonal selection of modified cells and quality control for maintenance of the pluripotent phenotype.

The Core designs and carries out custom research for investigators who do not have the experience or the infrastructure necessary to complete these experiments. These involve the production of 2D and 3D differentiated cells or the testing of small molecules and other reagents for biological effects or toxicity.

The Core provides consultation on any area of pluripotent stem cell research and training in all technologies described above.

If you received any services and/or used instruments within this core facility to generate data for your manuscript or grant submissions, please use the following examples to help format your acknowledgment:

• "The authors acknowledge the Stem Cell Research Core Facility at the University of Texas at San Antonio for support during this work."
• "The authors would like to thank Dr. Christopher Navara (Stem Cell Research Core Facility, University of Texas at San Antonio) for technical support.